Open AccessIsolation of hydrogenotrophic methanogenic archaea from a subtropical paddy field
Author(s) -
Adachi Katsuki
Publication year - 1999
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1999.tb00637.x
Subject(s) - biology , methanobacterium , 16s ribosomal rna , euryarchaeota , paddy field , methanogen , straw , botany , veterinary medicine , bacteria , microbiology and biotechnology , zoology , agronomy , archaea , medicine , genetics
A total of 347 blue‐autofluorescent colonies on roll tube cultures were transferred individually for isolation in order to examine methanogenic flora in a subtropical paddy field after application of organic matter with three treatments (control soil, straw‐amended soil, and cellulose‐amended soil). Two hundred and three strains showed a positive reaction for methane production. However, only 36 strains were found to be pure. Among the 36 pure methanogenic isolates, 19 utilized H 2 ‐CO 2 as sole source for energy and carbon for growth, 17 utilized H 2 ‐CO 2 and formate, and none utilized methanol and acetate. All 36 isolates were rods distinguishable into three groups by cell morphology: group 1, irregularly crooked long rods; group 2, gently curved long (or very long) rods; and group 3, relatively straight long (or very long) rods. Three isolates, strains 3544‐4 and 6544‐9 of group 2 and 5544‐3 of group 3, were identified as Methanobacterium spp. by partial sequencing analysis of 16S rRNA. The partial 16S rRNA sequence of strain 6544‐9 was identical to that of Methanobacterium bryantii . The other three isolates which belonged to group 1, strains 3543‐10, 3544‐9, and 6132‐1, seemed to represent a new species within the genus Methanobacterium or probably Methanobrevibacter . Each of the pure isolates from control and straw‐amended soil belonged to group 1. It is suggested that the group 1 methanogens are characterized as a geographically interesting indigenous type in the subtropical paddy field.