Open AccessComparative performance of enterobacterial repetitive intragenic consensus‐polymerase chain reaction and lipopolysaccharide electrophoresis for the identification of Bradyrhizobium sp. ( Lotus ) strains
Author(s) -
Santamarı́a M,
Agius F,
Monza J,
GutiérrezNavarro A.M,
Corzo J
Publication year - 1999
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1999.tb00571.x
Subject(s) - biology , polymerase chain reaction , lotus , dna profiling , typing , bradyrhizobium , genomic dna , genetics , microbiology and biotechnology , bacteria , dna , gene , rhizobium , botany
We compared two methods for typing bacterial strains: electrophoretic lipopolysaccharide profiling and genomic fingerprinting by enterobacterial repetitive intragenic consensus‐polymerase chain reaction. Our aim was to assess the relative utility of these techniques for identification of bradyrhizobia. A collection of Uruguayan Bradyrhizobium strains isolated from Lotus subbiflorus was selected to test both techniques in terms of their discriminating ability and ease of use. Both techniques were found to be equally discriminating and they classified the samples in the same way, although each method ascribed two strains to different groups. Genomic profiling of some strains required previous DNA purification, whereas this was found to be unnecessary for others. Lipopolysaccharide profiling was found to be easier and cheaper to perform, but was not useful for determination of the genetic relationship among the strains.