Open AccessFusarium udum is resistant to the mycolytic activity of a biocontrol strain of Bacillus subtilis AF 1
Author(s) -
Harish S.,
Manjula K.,
Podile A.R.
Publication year - 1998
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1998.tb00490.x
Subject(s) - bacillus subtilis , biology , chlamydospore , conidiation , mycelium , microbiology and biotechnology , chitinase , spore , inoculation , fusarium , biological pest control , strain (injury) , chitin , botany , horticulture , bacteria , mutant , biochemistry , anatomy , genetics , chitosan , gene
In vitro interaction of the pigeonpea wilt pathogen Fusarium udum and a biocontrol strain of Bacillus subtilis AF 1 showed that the fungus forms chlamydospore‐like structures and increases vacuolation, when both cultures are simultaneously inoculated into potato dextrose broth. If B. subtilis AF 1 was inoculated after 24 h of growth of F. udum , chlamydospores were not formed and regular conidiation was observed. The growth of F. udum was significantly affected in the presence of AF 1 in terms of dry weight. Extracellular proteins of B. subtilis AF 1 reduced the growth of F. udum in proportion to the concentration of the protein precipitate. Cell‐free filtrates of B. subtilis AF 1 prepared from chitin‐containing medium showed chitinase activity. The formation of chlamydospore‐like structures and vacuolated portions in mycelium of F. udum in the presence of AF 1 suggests that F. udum has a mechanism to tolerate mycolytic activity and grows slowly with regular conidiation.