Open AccessSlow‐growing and oligotrophic soil bacteria phylogenetically close to Bradyrhizobium japonicum
Author(s) -
Saito Akihiro,
Mitsui Hisayuki,
Hattori Reiko,
Minamisawa Kiwamu,
Hattori Tsutomu
Publication year - 1998
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1998.tb00480.x
Subject(s) - biology , bradyrhizobium japonicum , bradyrhizobium , 16s ribosomal rna , nitrogen fixation , botany , bacteria , rhizobiaceae , ribosomal dna , ribosomal rna , phylogenetic tree , microbiology and biotechnology , genetics , symbiosis , gene
Eleven isolates of slow‐growing oligotrophic bacteria from grassland soil were found to be closely related by partial 16S rRNA sequence similarity and many common taxonomic traits. Analysis of full 16S rRNA gene sequences of four representative isolates and Agromonas oligotrophica S58 indicated that they were more closely related to Bradyrhizobium japonicum , a symbiotic nitrogen‐fixing bacterium, (similarity values: 98.1–98.8%) than other strains such as Bradyrhizobium elkanii , Nitrobacter spp., Rhodopseudomonas palustris , and Afipia spp. This result was supported by analysis of phenotypic traits and DNA‐DNA hybridization analysis. No strain showed hybridization to nodD 1 YABC of B. japonicum , and only strain G14130 exhibited hybridization to nifDK ‐ and hupSL ‐specific DNA. These latter genotypes are involved in the phenotypes of nodulation and nitrogen fixation under microaerobic conditions. These results suggest that the isolates possess a unique phylogenetic position since they are closely related to B. japonicum though they do not have characteristics of symbiotic nitrogen fixation.