Open AccessPhenotypic characterization of natural populations of Fusarium oxysporum in relation to genotypic characterization
Author(s) -
Steinberg Christian,
Edel Véronique,
Gautheron Nadine,
Abadie Catherine,
Vallaeys Tatiana,
Alabouvette Claude
Publication year - 1997
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1997.tb00424.x
Subject(s) - biology , fusarium oxysporum , compatibility (geochemistry) , genotype , botany , natural population growth , population , genetics , gene , demography , geochemistry , sociology , geology
Sixty strains of Fusarium oxysporum were characterized by (i) in vitro growth parameter estimates on four carbon sources and (ii) vegetative compatibility grouping. Growth was assessed by measuring optical density changes of fungal cultures in microtiter plates. Principal component analyses of growth parameter estimates permitted a good discrimination of the isolates on the four carbon sources and revealed a high level of diversity within populations of F. oxysporum . The 60 strains were assigned to 40 vegetative compatibility groups. Strains grouped in a given vegetative compatibility group had similar growth parameters. Trophic characterization and vegetative compatibility groups were compared to a genotypic characterization previously performed on this collection. Trophic characterization and vegetative compatibility grouping were more discriminating than the genotypic characterization. The three characterization methods suggested that vegetative compatibility groups could be considered the population unit among natural F. oxysporum populations.