Open AccessTwo temperature optima of methane production in a typical soil of the Elbe river marshland
Author(s) -
Wagner Dirk,
Pfeiffer EvaMaria
Publication year - 1997
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1997.tb00366.x
Subject(s) - calcareous , mesophile , methane , incubation , anoxic waters , biology , marsh , methanogenesis , environmental chemistry , bacteria , ecology , wetland , botany , chemistry , biochemistry , genetics
The soil of the Elbe river marshland was classified as calcareous freshwater marsh (USDA: typic fluvaquent). Methanogenic bacteria were found in oxic as well as in anoxic soil layers. Most probable number counts varied from 1.4×10 2 to 2.5×10 6 bacteria g −1 fresh weight. Methane production at different temperatures (5°C, 10°C, 15°C, 20°C) was studied with undisturbed soil cores. The highest methane production rates were measured at 10°C (0.31 nmol h −1 (g d.w.) −1 ) and 20°C (0.58 nmol h −1 (g d.w.) −1 ). Incubation experiments with disturbed soil material showed two optima of methane production at 10°C and in the mesophilic temperature range. Experiments with different substrates indicate that at 10°C acetate serves as substrate while at higher temperatures hydrogen is the main energy source for methanogens.