Open AccessThe marine picoplankter Synechococcus sp. WH 7803 exhibits an adaptive response to restricted iron availability
Author(s) -
Chadd Helen E.,
Joint Ian R.,
Mann Nicholas H.,
Carr Noel G.
Publication year - 1996
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1996.tb00334.x
Subject(s) - biology , synechococcus , cytoplasm , methionine , biochemistry , chelation , amino acid , cyanobacteria , bacteria , chemistry , genetics , organic chemistry
Laboratory cultures of marine Synechococcus sp. WH 7803 were grown under conditions of restricted iron availability. The culture medium was adjusted to restrict iron availability: (i) by adding the iron chelator EDDA; (ii) by omitting iron; and (iii) by omitting both iron and EDTA. An adaptive response was observed to these iron‐restricted conditions, including a decrease in cellular phycoerythrin and synthesis of a 36 kDa polypeptide in [ 35 S]methionine radiolabelled whole cell lysates separated by SDS‐PAGE. The polypeptide was synthesized within 48 h of transferring exponential phase cells to the iron‐restricted medium. The protein was localized to the cell membranes and not the cytoplasmic fraction.