Open AccessConfirmation of ‘aerobic denitrification’ in batch cultures, using gas chromatography and 15 N mass spectrometry
Author(s) -
Robertson Lesley A.,
Dalsgaard Tage,
Revsbech NielsPeter,
Kuenen J.Gijs
Publication year - 1995
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1995.tb00168.x
Subject(s) - denitrifying bacteria , alcaligenes , denitrification , biology , aerobic denitrification , pseudomonas , microbiology and biotechnology , alcaligenes faecalis , mass spectrometry , chromatography , gas chromatography , pseudomonadaceae , bacteria , nitrogen , chemistry , organic chemistry , genetics
Because of a revival in the controversy surrounding ‘aerobic denitrification’, especially in relation to Thiosphaera pantotropha , activity in aerobic batch cultures was evaluated using gas chromatography and mass spectrometry after the addition of 15 N‐labelled NH 4 + and NO 2 − . Aerobic denitrifying activity in T. pantotropha was present, but only at about 10% of the originally‐reported levels. The activity of ‘ Pseudomonas denitrificans ’ was similar to previously‐reported values. Alcaligenes faecalis showed significant aerobic denitrifying activity, producing almost equivalent amounts of N 2 and N 2 O. An unidentified pseudomonad, isolate G4, presumably requires anoxia for enzyme activity as it did not denitrify aerobically, even though it has a constitutive denitrifying pathway.