Open AccessEffect of addition of rhamnolipid biosurfactants or rhamnolipid‐producing Pseudomonas aeruginosa on phenanthrene mineralization in soil slurries
Author(s) -
Providenti Miguel A.,
Flemming Cecily A.,
Lee Hung,
Trevors Jack T.
Publication year - 1995
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1995.tb00123.x
Subject(s) - phenanthrene , mineralization (soil science) , rhamnolipid , slurry , loam , creosote , environmental chemistry , soil microbiology , biology , chemistry , soil water , environmental engineering , environmental science , pseudomonas aeruginosa , bacteria , ecology , genetics
The effect of Pseudomonas aeruginosa UG2 biosurfactants or UG2 inocula on phenanthrene mineralization in uninoculated nonsterile soil slurries and slurries inoculated with the phenanthrene‐mineralizing Pseudomonas sp. UG14r was investigated. In sandy loam and silt loam slurries amended with phenanthrene, inoculation with UG14r alone or in co‐culture with UG2Lr reduced the lag period before onset of phenanthrene mineralization by 1 week. The total amount mineralized after 5 weeks was lower or not significantly different from the uninoculated control slurries. Inoculation with P. aeruginosa UG2Lr alone did not improve phenanthrene mineralization. In creosote‐contaminated soil slurries, no lag period in phenanthrene mineralization was observed in any treatment. After 4 weeks, the greatest extent of mineralization was observed in creosote‐contaminated soil slurries inoculated with the UG14r‐UG2Lr co‐culture and UG14r alone. In sandy loam and silt loam soil slurries inoculated with Pseudomonas sp. UG14r, addition of UG2 rhamnolipid biosurfactants (100 to 400 mg rhamnose equivalents (RE) · l −1 slurry) inhibited phenanthrene mineralization by 10 to 15%. Mineralization was also inhibited in uninoculated sandy loam slurries. In creosote‐contaminated soil slurries inoculated with Pseudomonas sp. UG14r, biosurfactants at 250 mg RE · l −1 slurry enhanced mineralization whereas 400 mg RE · l −1 had no effect, compared to unamended slurries. In uninoculated creosote‐contaminated soil slurries, UG2 biosurfactants at 250 and 400 mg RE · l −1 slurry enhanced mineralization, compared to unamended slurries.