Open AccessStability and conjugal transfer kinetics of a TOL plasmid in Pseudomonas aeruginosa PAO 1162
Author(s) -
Smets Barth F.,
Rittmann Bruce E.,
Stahl David A.
Publication year - 1994
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1994.tb00256.x
Subject(s) - biology , pseudomonadales , pseudomonas putida , pseudomonas aeruginosa , plasmid , pseudomonadaceae , microbiology and biotechnology , strain (injury) , pseudomonas , kinetics , bacteria , biochemistry , dna , anatomy , genetics , physics , quantum mechanics
Batch mating experiments were employed to study the kinetics of the conjugal transfer of a TOL plasmid, using the transconjugant strain Pseudomonas aeruginosa PAO 1162 (TOL) as the plasmid donor and Pseudomonas putida PB 2442 and Pseudomonas aeruginosa PAO 1162N as the plasmid recipients. Transfer rates from PAO 1162 (TOL) to PAO 1162N and PB 2442 measured for exponentially grown PAO 1162 (TOL) were 1.81 × 10 −14 (standard error (S.E.) 1.25 × 10 −15 ) ml·cell −1 min −1 and 3.32 × 10 −13 (S.E. 4.42 × 10 −14 ) ml·cell −1 min −1 , respectively. The instability of the TOL plasmid in PAO 1162 (TOL) was evaluated under conditions that were non‐selective for maintenance of the TOL catabolic functions. The measured rates of instability were 6.7 10 −6 to 8.3 10 −6 min −1 , and the loss of the catabolic functions was mainly caused by structural instability of the plasmid.