Open AccessTransposon Tn5403, a mobilization‐helper element: Complete nucleotide sequence and distribution in aquatic strains
Author(s) -
Rinkel Monique,
Hubert JeanClaude,
Roux Brigitte,
Lett MarieClaire
Publication year - 1994
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1994.tb00233.x
Subject(s) - biology , transposable element , dna transposable elements , mobilization , nucleic acid sequence , sequence (biology) , distribution (mathematics) , genetics , element (criminal law) , computational biology , genome , dna , gene , law , mathematical analysis , mathematics , political science
Tn 5403 , a new transposable element, was found in a Klebsiella pneumoniae strain (KIIIA) isolated from an aquatic environment following a pollution accident. It was identified by its ability to mobilize ori T‐deleted recombinant plasmids and thus can be proposed as a ‘helper’ element participating in the transfer mechanisms of non‐conjugative plasmids. Nucleotides sequence analysis shows that the element consists of 3663 bp. Two open reading frames (ORFs) are found: one ( tnp A) encoding a putative transposase and the other ( tnp R) a putative resolvase. The two ORFs are transcribed in opposite directions and are separated by a putative recombination site (res). Results obtained with a specific probe suggest that Tn 5403 or a similar element is present in different Enterobacteriaceae strains isolated from the same river three years after the isolation of the Tn 5403 ‐harbouring KIIIA strain.