Open AccessPCR‐amplification of mixed 16S rRNA genes from an anaerobic, cyanide‐degrading consortium
Author(s) -
Britschgi Theresa B.,
Fallon Robert D.
Publication year - 1994
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1994.tb00069.x
Subject(s) - biology , 16s ribosomal rna , polymerase chain reaction , gene , restriction fragment length polymorphism , ribosomal rna , primer (cosmetics) , genetics , microbiology and biotechnology , chemistry , organic chemistry
The microorganisms participating in the anaerobic biodegradation of cyanide were characterized using 16S rRNA genes as genetic markers of diversity. Segments of mixed population 16S rRNA genes were amplified using the polymerase chain reaction (PCR) and prokaryote‐specific amplification primers. Restriction fragment length polymorphism (RFLPs) and screening with the 926f universal sequencing primer were used to categorized the cloned PCR products. Six unique prokaryote sequence were obtained, including four similar to methanogens and two similar to Gram‐positive eubacteria.