Open AccessSurvival of the temperate actinophage φC31 and Streptomyces lividans in soil and the effects of competition and selection on lysogens
Author(s) -
Marsh P.,
Toth I.K.,
Meijer M.,
Schilhabel M.B.,
Wellington E.M.H.
Publication year - 1993
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1993.tb00046.x
Subject(s) - biology , thiostrepton , lysogen , microcosm , microbiology and biotechnology , lysogenic cycle , temperateness , spore , population , botany , ecology , genetics , gene , escherichia coli , bacteriophage , ribosome , rna , demography , sociology
Lysogenic infections were demonstrated in microcosms of sterile soil inoculated with Streptomyces lividans and the φC31 derivative, KC301, in free state or via lysogenized hosts. Intermittent soil mixing caused liberation of KC301 due to lysis of germinating lysogenized and uninfected spores. The presence of lysogenized host ensured that KC301 was maintained at a constant density. The lysogen S. lividans TK24 (KC301) achieved a population density lower than that of its non‐lysogenized counterpart. Thiostrepton in the soil did not select for the thiostrepton resistance gene KC301. The long‐term survival in soil of a temperate actinophage was demonstrated.