Open AccessIsolation and verification of anatoxin‐a producing clones of Anabaena flos‐aquae (Lyngb.) de Breb. from a eutrophic lake
Author(s) -
Kangatharalingam N.,
Priscu John C.
Publication year - 1993
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1993.tb00024.x
Subject(s) - biology , anabaena , trichome , clone (java method) , cyanobacteria , flos , botany , aphanizomenon , microbiology and biotechnology , bacteria , genetics , biochemistry , gene , rutin , antioxidant
We present a technique to isolate and confirm anatoxin‐a producing clones (single trichome‐isolates) of Anabaena flos‐aquae (Lyngb.) de Breb. from blooms of this cyanobacterium. A single trichome is isolated from a field sample and grown in ASM medium. Single trichomes are then isolated from this culture and grown in ASM medium to produce single clone cultures. Mouse bioassay, and thin‐layer chromatography (TLC) using purified anatoxin‐a as reference is then used to confirm the anatoxin‐a producing clones. Using this methodology, Anabaena flos‐aquae samples collected during July 1991 from Hebgen Lake, Montana, were found to contain only 8.7% anatoxin‐a producing clones. This minor proportion of anatoxin‐a producing clones apparently accounts for the anatoxin‐a produced by the entire population of A. flos‐aquae . Our technique is simple and reproducible. A selected clone of A. flos‐aquae that produces anatoxin‐a and one that does not produce anatoxin‐a were deposited in the UTEX culture collection, University of Texas at Austin.