Open AccessGAL promoter‐driven heterologous gene expression in S accharomyces cerevisiae Δ strain at anaerobic alcoholic fermentation
Author(s) -
Ahn Jungoh,
Park KyungMin,
Lee Hongweon,
Son YeoJin,
Choi EuiSung
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.12009.x
Subject(s) - biology , saccharomyces cerevisiae , heterologous , mutant , yeast , promoter , fermentation , ethanol fermentation , heterologous expression , gene , biochemistry , microbiology and biotechnology , gene expression , recombinant dna
The removal of G al80 protein by gene disruption turned into efficient GAL promoter‐driven heterologous gene expression under anaerobic alcoholic fermentation of S accharomyces cerevisiae . Using lipase B from C andida antarctica as a reporter, the relative strength of GAL10 promoter ( P GAL10 ) in Δgal80 mutant that does not require galactose as an inducer was compared to those of ADH1 , PDC1 , and PGK promoters, which have been known to work well anaerobically in actively fermenting yeast cells under high glucose concentration. P GAL10 in the Δgal80 mutant showed 0.8‐fold ( ADH1 ), fourfold ( PDC1 ), and 50‐fold ( PGK ) in promoter strength.