Open AccessQuantitative aerobic physiology of the yeast D ekkera bruxellensis , a major contaminant in bioethanol production plants
Author(s) -
Leite Fernanda Cristina Bezerra,
Basso Thiago Olitta,
Pita Will de Barros,
Gombert Andreas Karoly,
Simões Diogo Ardaillon,
Morais Marcos Antonio
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.12007.x
Subject(s) - chemostat , yeast , biology , sucrose , biomass (ecology) , biofuel , food science , ethanol fuel , biochemistry , ethanol , fermentation , microbiology and biotechnology , bacteria , ecology , genetics
D ekkera bruxellensis has been described as the major contaminant yeast of industrial ethanol production, although little is known about its physiology. The aim of this study was to investigate the growth of this yeast in diverse carbon sources and involved conducting shake‐flask and glucose‐ or sucrose‐limited chemostats experiments, and from the chemostat data, the stoichiometry of biomass formation during aerobic growth was established. As a result of the shake‐flask experiments with hexoses or disaccharides, the specific growth rates were calculated, and a different behavior in rich and mineral medium was observed concerning to profile of acetate and ethanol production. In C ‐limited chemostats conditions, the metabolism of this yeast was completely respiratory, and the biomass yields reached values of 0.62 gDW gS −1 . In addition, glucose pulses were applied to the glucose‐ or sucrose‐limited chemostats. These results showed that D . bruxellensis has a short‐term C rabtree effect. While the glucose pulse was at the sucrose‐limited chemostat, sucrose accumulated at the reactor, indicating the presence of a glucose repression mechanism in D . bruxellensis .