Open AccessPisiferdiol restores the growth of a mutant yeast suffering from hyperactivated C a 2+ signalling through calcineurin inhibition
Author(s) -
Aburai Nobuhiro,
Yoshida Jun,
Kobayashi Miki,
Mizunuma Masaki,
Ohnishi Motoko,
Kimura Kenichi
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.12003.x
Subject(s) - calcineurin , biology , yeast , mutant , microbiology and biotechnology , biochemistry , medicine , gene , transplantation
In the course of our screening program for a new inhibitor of the C a 2+ signalling pathway using mutant yeast [ S accharomyces cerevisiae ( zds1 Δ erg3 Δ pdr1 Δ pdr3 Δ)], a mouse PP 2 C α activator, pisiferdiol, isolated from C hamaecyparis pisifera , was found to alleviate the C a 2+ signal‐mediated growth inhibition. Pisiferdiol showed growth inhibition activity against the mpk1 Δ strain compared with the cnb1 Δ strain and induced L i + sensitivity to the wild‐type strain, indicating that it suppresses the calcineurin pathway in the yeast. However, the L i + sensitivity to ptc1 Δ strain by pisiferdiol was diminished. Pisiferdiol showed growth restored activity in the zds1 Δ strain without immunophilins F kb1p or C ph1p, and in the pmc1 Δ strain. It inhibited calcineurin‐induced expression in the reporter gene assay and decreased the protein expression ( W estern blots) of calcineurin ( C nb1p) in addition to a decrease of S we1p and phosphorylation of C dc28p in the mutant yeast. These results showed that pisiferdiol could suppress indirectly the action of calcineurin and restored the growth inhibition of the mutant yeast through P tc1p activation.