Open AccessA complete sequence of S accharomyces paradoxus mitochondrial genome that restores the respiration in S . cerevisiae
Author(s) -
Procházka Emanuel,
Franko Filip,
Poláková Silvia,
Sulo Pavol
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.00833.x
Subject(s) - biology , genetics , saccharomyces cerevisiae , mitochondrial dna , gene , intron , ribosomal rna , transfer rna , group i catalytic intron , ribosomal protein , genome , ribosome , rna , ribozyme
We determined the complete sequence of 71 355‐bp‐long mitochondrial genome from S accharomyces paradoxus entirely by direct sequencing of purified mitochondrial DNA (mt DNA ). This mt DNA possesses the same features as its close relative S accharomyces cerevisiae – A + T content 85.9%, set of genes coding for the three components of cytochrome oxidase, cytochrome b, three subunits of ATP ase, both ribosomal subunits, gene for ribosomal protein, rnpB gene, t RNA package (24) and yeast genetic code. Genes are interrupted by nine group I and group II introns, two of which are in positions unknown in S . cerevisiae , but recognized in S accharomyces pastorianus . The gene products are related to S . cerevisiae, and the identity of amino acid residues varies from 100% for cox2 to 83% for rps3 . The remarkable differences from S . cerevisiae are (1) different gene order (translocation of trnF ‐ trnT1 ‐ trnV ‐ cox3 ‐ trnfM ‐ rnpb ‐ trnP and transposition of trnW ‐ rns ), (2) occurrence of two unusual GI introns, (3) eight active ori elements, and (4) reduced number of GC clusters and divergent intergenic spacers. Despite these facts, the sequenced S . paradoxus mt DNA introduced to S . cerevisiae was able to support the respiratory function to the same extent as the original mt DNA s.