Open AccessNutritional requirements of the BY series of S accharomyces cerevisiae strains for optimum growth
Author(s) -
Hanscho Michael,
Ruckerbauer David E.,
Chauhan Neha,
Hofbauer Harald F.,
Krahulec Stefan,
Nidetzky Bernd,
Kohlwein Sepp D.,
Zanghellini Juergen,
Natter Klaus
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.00830.x
Subject(s) - auxotrophy , biology , saccharomyces cerevisiae , yeast , biochemistry , serine , amino acid , inositol , threonine , exponential growth , escherichia coli , gene , phosphorylation , mathematical analysis , receptor , mathematics
Among the vast variety of S accharomyces cerevisiae strains, the BY family is particularly important because the widely used deletion collections are based on this background. Here we demonstrate that some standard growth media recipes require substantial modifications to provide optimum growth conditions for auxotrophic BY strains and to avoid growth arrest before glucose is depleted. In addition to the essential supplements that are required to satisfy auxotrophic requirements, we found the four amino acids phenylalanine, glutamic acid, serine, and threonine to be indispensable for optimum growth, despite the fact that BY is ‘prototrophic’ for these amino acids. Interestingly, other widely used S . cerevisiae strains, such as strains of the CEN . PK family, are less sensitive to lack of the described supplements. Furthermore, we found that the concentration of inositol in yeast nitrogen base is too low to support fast proliferation of yeast cultures until glucose is exhausted. Depletion of inositol during exponential growth induces characteristic changes, namely a decrease in glucose uptake and maximum specific growth rate, increased cell size, reduced viability, and accumulation of lipid storage pools. Thus, several of the existing growth media recipes need to be revised to achieve optimum growth conditions for BY ‐derived strains.