Open AccessEndocytosis inhibition during H 2 O 2 ‐induced apoptosis in yeast
Author(s) -
Pereira Clara,
Bessa Cláudia,
Saraiva Lucília
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.00825.x
Subject(s) - endocytosis , biology , endocytic cycle , microbiology and biotechnology , apoptosis , yeast , saccharomyces cerevisiae , fragmentation (computing) , programmed cell death , dna fragmentation , oxidative stress , receptor mediated endocytosis , cell , biochemistry , ecology
Yeast revealed to be a versatile organism for studying endocytosis. Here, inhibition of endocytosis by H 2 O 2 and its correlation with apoptotic cell death were ascertained in S accharomyces cerevisiae . We found that H 2 O 2 causes alterations in vacuolar morphology and a concentration‐dependent inhibition of endocytosis. We also found that H 2 O 2 ‐induced endocytosis inhibition is a reversible process that occurs in the early phase of the apoptotic cascade, preceding chromatin condensation and DNA fragmentation. Additionally, mutants affecting early steps of the endocytic pathway display sensitivity to H 2 O 2 . As endocytosis inhibition was also observed with acetic acid, it may be a broader cellular dysfunction of oxidative stress‐induced toxicity in yeast.