Open AccessAccumulation of phosphate and polyphosphate by C ryptococcus humicola and S accharomyces cerevisiae in the absence of nitrogen
Author(s) -
Breus Natalia A.,
Ryazanova Lubov P.,
Dmitriev Vladimir V.,
Kulakovskaya Tatiana V.,
Kulaev Igor S.
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.00812.x
Subject(s) - polyphosphate , biology , saccharomyces cerevisiae , phosphate , cryptococcus , microbiology and biotechnology , nitrogen , biochemistry , yeast , chemistry , organic chemistry
The search for new phosphate‐accumulating microorganisms is of interest in connection with the problem of excess phosphate in environment. The ability of some yeast species belonging to ascomycetes and basidiomycetes for phosphate ( P i ) accumulation in nitrogen‐deficient medium was studied. The ascomycetous S accharomyces cerevisiae and K uraishia capsulata and basidiomycetous C ryptococcus humicola , Cryptococcus curvatus , and P seudozyma fusiformata were the best in P i removal. The cells of C ryptococcus humicola and S . cerevisiae took up 40% P i from the media containing P i and glucose (5 and 30 mM, respectively), and up to 80% upon addition of 5 mM MgSO 4 . The cells accumulated P i mostly in the form of polyphosphate ( P oly P ). In the presence of Mg 2+ , the content of P oly P with longer average chain length increased in both yeasts; they both had numerous inclusions fluorescing in the yellow region of the spectrum, typical of DAPI ‐ P oly P complexes. Among the yeast species tested, C ryptococcus humicola is a new promising model organisms to study phosphorus removal from the media and biomineralization in microbial cells.