Open AccessStress adaptation of Saccharomyces cerevisiae as monitored via metabolites using two‐dimensional NMR spectroscopy
Author(s) -
Kang Woo Young,
Kim Seol Hyun,
Chae Young Kee
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.00811.x
Subject(s) - saccharomyces cerevisiae , metabolite , yeast , biology , metabolomics , computational biology , metabolite profiling , nuclear magnetic resonance spectroscopy , saccharomyces , metabolic adaptation , biochemistry , metabolism , bioinformatics , chemistry , stereochemistry
Many studies on yeast metabolism are focused on its response to specific stress conditions because the results can be extended to the human medical issues. Most of those works have been accomplished through functional genomics studies. However, these changes may not show a linear correlation with protein or metabolite levels. For many organisms including yeast, the number of metabolites is far fewer than that of genes or gene products. Thus, metabolic profiling can provide a simpler yet efficient snapshot of the system's physiology. Metabolites of S accharomyces cerevisiae under various stresses were analyzed and compared with those under the normal, unstressed growth conditions by two‐dimensional NMR spectroscopy. At least 31 metabolites were identified for most of the samples. The levels of many identified metabolites showed significant increase or decrease depending on the nature of the stress. The statistical analysis produced a holistic view: different stresses were clustered and isolated from one another with the exception of high pH , heat, and oxidative stresses. This work could provide a link between the metabolite profiles and mRNA or protein profiles under representative and well‐studied stress conditions.