Open AccessEvolutionary engineering of S accharomyces cerevisiae for efficient aerobic xylose consumption
Author(s) -
Scalcinati Gionata,
Otero José Manuel,
Vleet Jennifer R.H.,
Jeffries Thomas W.,
Olsson Lisbeth,
Nielsen Jens
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.00808.x
Subject(s) - xylose , xylose metabolism , saccharomyces cerevisiae , metabolic engineering , xylitol , yeast , biochemistry , biology , pichia stipitis , pentose , biomass (ecology) , biorefinery , ethanol fuel , fermentation , microbiology and biotechnology , food science , biofuel , enzyme , agronomy
Industrial biotechnology aims to develop robust microbial cell factories, such as S accharomyces cerevisiae , to produce an array of added value chemicals presently dominated by petrochemical processes. Xylose is the second most abundant monosaccharide after glucose and the most prevalent pentose sugar found in lignocelluloses. Significant research efforts have focused on the metabolic engineering of S . cerevisiae for fast and efficient xylose utilization. This study aims to metabolically engineer S . cerevisiae , such that it can consume xylose as the exclusive substrate while maximizing carbon flux to biomass production. Such a platform may then be enhanced with complementary metabolic engineering strategies that couple biomass production with high value‐added chemical. S accharomyces cerevisiae , expressing xylose reductase, xylitol dehydrogenase and xylulose kinase, from the native xylose‐metabolizing yeast P ichia stipitis , was constructed, followed by a directed evolution strategy to improve xylose utilization rates. The resulting S . cerevisiae strain was capable of rapid growth and fast xylose consumption producing only biomass and negligible amount of byproducts. Transcriptional profiling of this strain was employed to further elucidate the observed physiology confirms a strongly up‐regulated glyoxylate pathway enabling respiratory metabolism. The resulting strain is a desirable platform for the industrial production of biomass‐related products using xylose as a sole carbon source.