Open AccessThe regulation of different metabolic pathways through the P al/ R im pathway in U stilago maydis
Author(s) -
FonsecaGarcía Citlali,
LeónRamírez Claudia G.,
RuizHerrera José
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.00805.x
Subject(s) - ustilago , biology , mutant , intracellular , metabolic pathway , microbiology and biotechnology , phenotype , fungus , gene , biochemistry , botany
One of the most important physicochemical factors that affect cell growth and development is p H , and living organisms have developed specific mechanisms to adapt to media with variable p H values. Most fungi posses a specific mechanism for such adaptation: the P al/ R im pathway. To analyze the different metabolic processes regulated by this pathway, and its possible relationships with other physiological regulatory mechanisms, we analyzed the phenotype of a mutant in the PALB / RIM13 gene of the phytopathogenic fungus U stilago maydis . The mutant displayed important alterations in the synthesis and organization of the cell wall and was affected in its response to stress, revealing its relationship with the MAPKC pathway involved in maintaining the integrity of the cell wall, and the stress response pathway, but not with the HOG pathway. An important observation was that the mutant, in contrast to the wild‐type strain, was unable to maintain a constant intracellular p H , suggesting that probably the main function of the P al/ R im pathway, in collaboration with other regulatory mechanisms, is to maintain a constant intracellular p H , despite the changes occurring in the environment.