Open AccessSolid‐state NMR spectroscopy reveals anomer specific transport of galactose in the milk yeast K luyveromyces lactis
Author(s) -
Fukasawa Toshio,
Abe Akio,
Nakamura Atsusi,
Horigome Miyako,
Naito Akira
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2012.00788.x
Subject(s) - kluyveromyces lactis , galactose , kluyveromyces , anomer , yeast , biochemistry , nuclear magnetic resonance spectroscopy , biology , glucose transporter , saccharomyces cerevisiae , stereochemistry , chemistry , endocrinology , insulin
Genetic evidence indicates that only the β‐anomer of galactose is transported to K luyveromyces lactis cells by galactose/glucose transporter H gt1p, and that aldose‐1‐epimerase encoded by GAL 10 is a prerequisite for growth on galactose. Minor aldose‐1‐epimerases other than G al10p also exist in K . lactis . Using a mutant defective in both aldose‐1‐epimerases, we show by solid‐state nuclear magnetic resonance spectroscopy that only β‐anomer is transported in the cell and stays without or with a slow rate of conversion to α‐anomer. Signals due to intracellular β‐galactose appeared at two positions, both of which were shifted towards higher magnetic fields than that of β‐galactose in aqueous solution, suggesting that incorporated galactose binds to cellular components, probably proteins.