Open AccessThe genome sequence of the wine yeast VIN 7 reveals an allotriploid hybrid genome with S accharomyces cerevisiae and S accharomyces kudriavzevii origins
Author(s) -
Borneman Anthony R.,
Desany Brian A.,
Riches David,
Affourtit Jason P.,
Forgan Angus H.,
Pretorius Isak S.,
Egholm Michael,
Chambers Paul J.
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2011.00773.x
Subject(s) - saccharomyces cerevisiae , biology , wine , genome , genetics , winemaking , ploidy , saccharomyces , yeast , whole genome sequencing , gene , food science
The vast majority of wine fermentations are performed principally by S accharomyces cerevisiae . However, there are a growing number of instances in which other species of S accharomyces play a predominant role. Interestingly, the presence of these other yeast species generally occurs via the formation of interspecific hybrids that contain genomic contributions from both S . cerevisiae and non‐ S . cerevisiae species. However, despite the large number of wine strains that are characterized at the genomic level, there remains limited information regarding the detailed genomic structure of hybrids used in winemaking. To address this, we describe the genome sequence of the thiol‐releasing commercial wine yeast hybrid VIN 7. VIN 7 is shown to be an almost complete allotriploid interspecific hybrid that is comprised of a heterozygous diploid complement of S . cerevisiae chromosomes and a haploid S accharomyces kudriavzevii genomic contribution. Both parental strains appear to be of European origin, with the S . cerevisiae parent being closely related to, but distinct from, the commercial wine yeasts QA 23 and EC 1118. In addition, several instances of chromosomal rearrangement between S . cerevisiae and S . kudriavzevii sequences were observed that may mark the early stages of hybrid genome consolidation.