Open AccessAnalysis of the biological activity of a novel 24‐membered macrolide JBIR ‐19 in S accharomyces cerevisiae by the morphological imaging program C al M orph
Author(s) -
Ohnuki Shinsuke,
Kobayashi Tomohide,
Ogawa Hayato,
Kozone Ikuko,
Ueda Junya,
Takagi Motoki,
Shinya Kazuo,
Hirata Dai,
Nogami Satoru,
Ohya Yoshikazu
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2011.00770.x
Subject(s) - biology , saccharomyces cerevisiae , yeast , entomopathogenic fungus , phenotype , botany , microbiology and biotechnology , gene , biochemistry , biological pest control , beauveria bassiana
To investigate the biological activity of a novel 24‐membered macrolide compound, JBIR ‐19, isolated from the culture broth of the entomopathogenic fungus M etarhizium sp. f E 61, morphological changes in yeast cells were examined using the automated image‐processing program C al M orph . Principal components analysis was used to elucidate dynamic changes in the phenotypes, revealing two independent effects of JBIR ‐19 in yeast cells: bud elongation and increased size of the actin region. Using a fitness assay, we identified the genes required for robust growth in the presence of JBIR ‐19. Among these were CCW12 , YLR111W , and DHH1 , which are also involved in abnormal bud morphology. Based on these results and others, we predict intracellular targets of JBIR ‐19 and its functional interactions.