Open AccessIdentification of RCN1 and RSA3 as ethanol‐tolerant genes in S accharomyces cerevisiae using a high copy barcoded library
Author(s) -
Anderson Michael J.,
Barker Sarah L.,
Boone Charlie,
Measday Vivien
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2011.00762.x
Subject(s) - saccharomyces cerevisiae , biology , yeast , wine , open reading frame , yeast in winemaking , fermentation , ethanol fermentation , genetics , gene , microbiology and biotechnology , biochemistry , food science , peptide sequence
S accharomyces cerevisiae ( S . cerevisiae ) encounters a multitude of stresses during industrial processes such as wine fermentation including ethanol toxicity. High levels of ethanol reduce the viability of yeast and may prevent completion of fermentation. The identification of ethanol‐tolerant genes is important for creating stress‐resistant industrial yeast, and S . cerevisiae genomic resources have been utilized for this purpose. We have employed a molecular barcoded yeast open reading frame ( MoBY ‐ ORF ) high copy plasmid library to identify ethanol‐tolerant genes in both the S . cerevisiae S 288 C laboratory and M 2 wine strains. We find that increased dosage of either RCN1 or RSA3 improves tolerance of S 288 C and M 2 to toxic levels of ethanol. RCN1 is a regulator of calcineurin, whereas RSA3 has a role in ribosome maturation. Additional fitness advantages conferred upon overproduction of RCN1 and RSA3 include increased resistance to cell wall degradation, heat, osmotic and oxidative stress. We find that the M 2 wine yeast strain is generally more tolerant of stress than S 288 C with the exception of translation inhibition, which affects M 2 growth more severely than S 288 C . We conclude that regulation of ribosome biogenesis and ultimately translation is a critical factor for S . cerevisiae survival during industrial‐related environmental stress.