Open AccessP kc1 and actin polymerisation activities play a role in ribosomal gene repression associated with secretion impairment caused by oxidative stress
Author(s) -
Mitjana Felipe V.,
Petkova Mima I.,
PujolCarrion Nuria,
TorreRuiz Maria Angeles
Publication year - 2011
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2011.00754.x
Subject(s) - biology , oxidative stress , psychological repression , ribosomal protein , secretion , actin , microbiology and biotechnology , saccharomyces cerevisiae , actin cytoskeleton , gene expression , hydrogen peroxide , gene , downregulation and upregulation , transcription (linguistics) , cytoskeleton , biochemistry , cell , rna , ribosome , linguistics , philosophy
In S accharomyces cerevisiae , the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the P kc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required for this function, because the cells blocked in actin polymerisation were unable to repress ribosomal gene transcription. Following the invertase secretion pattern, we hypothesize that oxidative stress induced by hydrogen peroxide could have affected the latter steps of secretion. This would explain why the P kc1 function was required to repress ribosomal biogenesis.