Open AccessDrug‐resistant cassettes for the efficient transformation of Candida guilliermondii wild‐type strains
Author(s) -
Millerioux Yoann,
Clastre Marc,
Simkin Andrew J.,
Courdavault Vincent,
Marais Emeline,
Sibirny Andriy A.,
Noël Thierry,
Crèche Joël,
GiglioliGuivarc'h Nathalie,
Papon Nicolas
Publication year - 2011
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2011.00731.x
Subject(s) - biology , complementation , cytosine deaminase , yeast , microbiology and biotechnology , transformation (genetics) , flucytosine , drug resistance , gene , selectable marker , genetics , computational biology , fluconazole , antifungal , phenotype , genetic enhancement
Candida guilliermondii is an opportunistic emerging fungal agent of candidiasis often associated with oncology patients. This yeast also remains an interesting biotechnological model for the industrial production of value‐added metabolites. The recent whole‐genome sequencing of the C. guilliermondii ATCC 6260 reference strain provides an interesting resource for elucidating new molecular events supporting pathogenicity, antifungal resistance and for exploring the potential of yeast metabolic engineering. In the present study, we designed an efficient transformation system for C. guilliermondii wild‐type strains using both nourseothricin‐ and hygromycin B‐resistant markers. To demonstrate the potential of these drug‐resistant cassettes, we carried out the disruption and the complementation of the C. guilliermondii FCY1 gene (which encodes cytosine deaminase) known to be associated with flucytosine sensitivity in yeast. These two new dominant selectable markers represent powerful tools to study the function of a large pallet of genes in this yeast of clinical and biotechnological interest.