Open AccessA fungicidal piperazine‐1‐carboxamidine induces mitochondrial fission‐dependent apoptosis in yeast
Author(s) -
Bink Anna,
Govaert Gilmer,
François Isabelle E.J.A.,
Pellens Klaartje,
Meerpoel Lieven,
Borgers Marcel,
Van Minnebruggen Geert,
Vroome Valérie,
Cammue Bruno P.A.,
Thevissen Karin
Publication year - 2010
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2010.00663.x
Subject(s) - biology , fis1 , yeast , mitochondrial fission , saccharomyces cerevisiae , apoptosis , mitochondrion , fission , microbiology and biotechnology , intracellular , schizosaccharomyces , function (biology) , biochemistry , schizosaccharomyces pombe , mitochondrial fusion , gene , mitochondrial dna , physics , quantum mechanics , neutron
To unravel the working mechanism of the fungicidal piperazine‐1‐carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase‐dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329‐induced yeast cell death.