Open AccessMulticopy suppression of oxidant‐sensitive eos1 mutation by IZH2 in Saccharomyces cerevisiae and the involvement of Eos1 in zinc homeostasis
Author(s) -
Nakamura Toshihide,
Takahashi Shunsuke,
Takagi Hiroshi,
Shima Jun
Publication year - 2010
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2010.00608.x
Subject(s) - biology , saccharomyces cerevisiae , gene , mutant , genetics , mutation , plasmid , phenotype , microbiology and biotechnology
EOS1 is required for tolerance to oxidative stress in Saccharomyces cerevisiae ; mutants are defective in the gene sensitive to hydrogen peroxide and tolerant to tunicamycin. To clarify the function of Eos1, we screened yeast genomic DNA libraries for heterologous genes that, when overexpressed from a plasmid, can suppress the hydrogen peroxide‐sensitive eos1 mutation. We identified one such gene, IZH2 , which has previously been reported to be a Zap1‐regulated gene. However, the EOS1 and IZH2 genes do not themselves appear to be functionally interchangeable. Double disruption of the EOS1 and IZH2 genes yielded a slow‐growth phenotype, suggesting that the two proteins are involved in related cellular processes. DNA microarray analysis revealed decreased expression of Zap1‐regulated genes in the eos1 ‐deletion mutant (Δ eos1 ). Thus, it is likely that Eos1 is involved in zinc homeostasis.