Open AccessGeotrichum siamensis sp. nov. and Geotrichum phurueaensis sp. nov., two asexual arthroconidial yeast species isolated in Thailand
Author(s) -
Kaewwichian Rungluk,
Yongmanitchai Wichien,
Srisuk Nantana,
Fujiyama Kazuhito,
Limtong Savitree
Publication year - 2010
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2009.00572.x
Subject(s) - geotrichum , biology , phylogenetic tree , botany , clade , strain (injury) , ribosomal rna , 16s ribosomal rna , bacteria , gene , genetics , anatomy
Two asexual arthroconidial yeast strains, TM3‐44 T and LYSM5 T , were isolated, respectively, from estuarine water in a mangrove forest and soil in a terrestrial forest in Thailand. Analysis of the D1/D2 domains of the large‐subunit rRNA gene sequences revealed that strain TM3‐44 T differed from the closest species in terms of pairwise sequence similarity, Dipodascus albidus , by 11.7% nucleotide substitutions, while strain LYSM5 T was closest to Galactomyces geotrichum with only 2.9% nucleotide substitutions. The phylogenetic tree further demonstrated that strain TM3‐44 T was at a distant position from the closest species, D. albidus , and other related species in the Dipodascus clade, while strain LYSM5 T clustered with G. geotrichum , it closest relative in the Galactomyces clade. The phenotypic characteristics of the two strains were typical of the genus Geotrichum . On the basis of the above findings, strain TM3‐44 T was assigned as a novel species of Geotrichum , for which the name Geotrichum siamensis sp. nov. is proposed. The type strain is TM3‐44 T (BCC 29903 T =NBRC 104880 T =CBS 10929 T ). Strain LYSM5 T represented another novel species of Geotrichum , which was named Geotrichum phurueaensis sp. nov. The type strain is LYSM5 T (BCC 34756 T =NBRC 105674 T =CBS 11418 T ).