Open AccessTorulaspora quercuum sp. nov. and Candida pseudohumilis sp. nov., novel yeasts from human and forest habitats
Author(s) -
Wang QiMing,
Xu Jianping,
Wang Huamin,
Li Juan,
Bai FengYan
Publication year - 2009
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2009.00567.x
Subject(s) - biology , internal transcribed spacer , strain (injury) , ribosomal rna , taxonomy (biology) , botany , microbiology and biotechnology , gene , genetics , anatomy
Strains XZ‐46A, XZ‐105, XZ‐129 and XZ‐281 T isolated from the oral cavities of healthy Tibetan volunteers were revealed to represent two novel ascomycetous yeast species by molecular taxonomic characterizations. Strain XZ‐281 T was most closely related to Candida humilis , but differed from the type strain of the species by eight (1.2%) substitutions in the 26S rRNA gene D1/D2 domain and by >100 (>20%) mismatches in the internal transcribed spacer (ITS) region. Strains XZ‐46A, XZ‐105 and XZ‐129 had identical or similar D1/D2 and ITS sequences with each other and with strain 17YF T isolated from a leaf of an oak tree ( Quercus sp.). The closest relative of this group was Torulaspora microellipsoides . They differed from the type strain of the species by five (0.9%) substitutions in the D1/D2 domain and >70 (>15%) mismatches in the ITS region. A sexual state was observed in strain 17YF T , but not in the other four oral strains. An anamorphic name Candida pseudohumilis sp. nov. is proposed for strain XZ‐281 T (=AS 2.3956 T =CBS 11404 T ) and a teleomorphic name Torulaspora quercuum sp. nov. is proposed for strain 17YF T (=AS 2.3768 T =CBS 11403 T ) and the other three oral strains.