Open AccessA phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY
Author(s) -
Gombault Aurélie,
Warringer Jonas,
Caesar Robert,
Godin Fabienne,
Vallée Béatrice,
Doudeau Michel,
Chautard Hélène,
Blomberg Anders,
Bénédetti Hélène
Publication year - 2009
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2009.00535.x
Subject(s) - biology , mutant , saccharomyces cerevisiae , phenotype , protease , gtpase , function (biology) , microbiology and biotechnology , carboxypeptidase , biochemistry , genetics , gene , enzyme
The Saccharomyces cerevisiae protein Tfs1p is known as a dual protein. On the one hand, it inhibits the carboxypeptidase Y protease, and on the other, it inhibits Ira2p, a GTPase‐activating protein of Ras. We managed to dissect precise areas of Tfs1p specifically involved in only one of those functions. Based on these data, specific Tfs1p point mutants affected in only one of these two functions were constructed. In order to obtain insights on the physiological role of these functions, systematic phenotypic tests were performed on strains expressing these specific Tfs1p mutants. The results obtained demonstrate that the inhibition of Ira2p by Tfs1p is the predominant function under the conditions tested.