Open AccessProtective effect of ions against cell death induced by acid stress in Saccharomyces
Author(s) -
Dos Santos Sant'Ana Gilzeane,
Da Silva Paes Lisvane,
Vieira Paiva Argentino F.,
Fietto Luciano Gomes,
Totola Antônio Helvécio,
Magalhães Trópia Maria J.,
Lemos Denise Silveira,
Lucas Cândida,
Fietto Juliana L. Rangel,
Brandão Rogelio L.,
Castro Ieso de Miranda
Publication year - 2009
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2009.00523.x
Subject(s) - saccharomyces cerevisiae , antiporter , biology , saccharomyces boulardii , yeast , osmotic shock , probiotic , microbiology and biotechnology , biochemistry , viability assay , vacuole , sodium , food science , cell , membrane , bacteria , chemistry , genetics , organic chemistry , cytoplasm , gene
Saccharomyces boulardii is a probiotic used to prevent or treat antibiotic‐induced gastrointestinal disorders and acute enteritis. For probiotics to be effective they must first be able to survive the harsh gastrointestinal environment. In this work, we show that S. boulardii displayed the greatest tolerance to simulated gastric environments compared with several Saccharomyces cerevisiae strains tested. Under these conditions, a pH 2.0 was the main factor responsible for decreased cell viability. Importantly, the addition of low concentrations of sodium chloride (NaCl) protected cells in acidic conditions more effectively than other salts. In the absence of S. boulardii mutants, the protective effects of Na + in yeast viability in acidic conditions was tested using S. cerevisiae Na + ‐ATPases ( ena1‐4 ), Na + /H + antiporter ( nha1 Δ) and Na + /H + antiporter prevacuolar ( nhx1 Δ) null mutants, respectively. Moreover, we provide evidence suggesting that this protection is determined by the plasma membrane potential, once altered by low pH and low NaCl concentrations. Additionally, the absence or low expression/activity of Ena proteins seems to be closely related to the basal membrane potential of the cells.