Open AccessImportance of the cytochrome P450 monooxygenase CYP52 family for the sophorolipid‐producing yeast Candida bombicola
Author(s) -
Van Bogaert Inge N.A.,
Demey Marjan,
Develter Dirk,
Soetaert Wim,
Vandamme Erick J.
Publication year - 2009
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2008.00454.x
Subject(s) - yeast , biology , monooxygenase , cytochrome p450 , downregulation and upregulation , gene , biochemistry , genetics , enzyme
Three cytochrome P450 monooxygenases belonging to the CYP52 family were isolated from the genome of the sophorolipid‐producing yeast Candida bombicola using degenerate PCR and genomic walking. One gene displayed high identity with the CYP52E members and was classified into this group ( CYP52E3 ), whereas the other genes belonged to new groups: CYP52M and CYP52N. CYP52E3 and CYP52N1 turned out to be of no relevance for sophorolipid production, but show clear upregulation when the yeast cells are grown on alkanes as the sole carbon source. On the other hand, CYP52M1 is clearly upregulated during sophorolipid synthesis and very likely takes part in sophorolipid formation.