Open AccessEffect of a carbon source on polyphosphate accumulation in Saccharomyces cerevisiae
Author(s) -
Vagabov Vladimir M.,
Trilisenko Ludmila V.,
Kulakovskaya Tatiana V.,
Kulaev Igor S.
Publication year - 2008
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2008.00420.x
Subject(s) - polyphosphate , fraction (chemistry) , ethanol , saccharomyces cerevisiae , biochemistry , phosphate , carbon fibers , biology , yeast , chromatography , chemistry , materials science , composite number , composite material
The cells of Saccharomyces cerevisiae accumulate inorganic polyphosphate (polyP) when reinoculated on a phosphate‐containing medium after phosphorus starvation. Total polyP accumulation was similar at cultivation on both glucose and ethanol. Five separate fractions of polyP: acid‐soluble fraction polyP1, salt‐soluble fraction polyP2, weakly alkali‐soluble fraction polyP3, alkali‐soluble fraction polyP4, and polyP5, have been obtained from the cells grown on glucose and ethanol under phosphate overplus. The dynamics of polyP fractions depend on a carbon source. The accumulation rates for fractions polyP2 and polyP4 were independent of the carbon source. The accumulation rates of polyP1 and polyP3 were higher on glucose, while fraction polyP5 accumulated faster on ethanol. As to the maximal polyP levels, they were independent of the carbon source for fractions polyP2, polyP3, and polyP4. The maximal level of fraction polyP1 was higher on glucose than on ethanol, but the level of fraction polyP5 was higher on ethanol. It was assumed that accumulation of separate polyP fractions has a metabolic interrelation with different energy‐providing pathways. The polyphosphate nature of fraction polyP5 was demonstrated for the first time by 31 P nuclear magnetic resonance spectroscopy, enzymatic assay, and electrophoresis.