Open AccessA novel gene, ecl1 + , extends the chronological lifespan in fission yeast
Author(s) -
Ohtsuka Hokuto,
Mita Satoka,
Ogawa Yuki,
Azuma Kenko,
Ito Hirokazu,
Aiba Hirofumi
Publication year - 2008
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2008.00379.x
Subject(s) - schizosaccharomyces pombe , biology , mutant , gene , plasmid , wild type , yeast , genetics , mutation , microbiology and biotechnology
We have identified a novel gene from Schizosaccharomyces pombe that we have named ecl1 + (extender of the chronological lifespan). When ecl1 + is provided on a high‐copy number plasmid, it extends the viability of both the Δ sty1 MAP kinase mutant and the wild‐type cells after entry into the stationary phase. ecl1 + encodes an 80‐amino acid polypeptide that had not been annotated in the current database. The ecl1 + ‐mRNA increases transiently when the growth phase is changed from the log phase to the stationary phase. The Ecl1 protein is localized in the nucleus. Calorie restriction extends the chronological lifespan of wild‐type and Δ ecl1 cells but not ecl1 + ‐overproducing cells. The Δ pka1 mutant shows little, if any, additional extension of viability when Ecl1 is overproduced. The ste11 + gene that is negatively controlled by Pka1 is up regulated when Ecl1 is overproduced. From these results we propose that the effect of Ecl1 overproduction may be mainly linked to and negatively affects the Pka1‐dependent pathway.