Open AccessRESEARCH ARTICLE: A transient proteasome activation is needed for acetic acid‐induced programmed cell death to occur in Saccharomyces cerevisiae
Author(s) -
Valenti Daniela,
Vacca Rosa Anna,
Guaragnella Nicoletta,
Passarella Salvatore,
Marra Ersilia,
Giannattasio Sergio
Publication year - 2008
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2008.00348.x
Subject(s) - mg132 , proteasome , saccharomyces cerevisiae , programmed cell death , biology , microbiology and biotechnology , yeast , acetic acid , proteasome inhibitor , apoptosis , cell , biochemistry
To gain further insight into the mechanism by which yeast programmed cell death (PCD) occurs, we investigated whether and how proteasome activity changes in Saccharomyces cerevisiae cells undergoing PCD as a result of treatment with acetic acid (AA‐PCD). We show that proteasome activation starts 60 min after AA‐PCD induction, with a maximum at 90 min, and decreases at 150 min. Moreover, cell survival measurements carried out in the absence or presence of MG132, which inhibits proteasome function, show that the inhibition of proteasome activity partially prevents AA‐PCD, thus indicating that a transient proteasome activation is needed for AA‐PCD to occur.