Open AccessAlzheimer's Aβ fused to green fluorescent protein induces growth stress and a heat shock response
Author(s) -
Caine Jo,
Sankovich Sonia,
Antony Helma,
Waddington Lynne,
Macreadie Peter,
Varghese Jose,
Macreadie Ian
Publication year - 2007
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2007.00285.x
Subject(s) - intracellular , yeast , biology , heat shock protein , green fluorescent protein , heat shock , alzheimer's disease , peptide , microbiology and biotechnology , fluorescence , biochemistry , disease , gene , medicine , physics , quantum mechanics
The 42 amino acid Alzheimer's Aβ peptide is involved in the progression of Alzheimer's disease. Here we describe the effects of intracellular Aβ, produced through its attachment to either end of a green fluorescent protein, in yeast. Cells producing Aβ exhibited a lower growth yield and a heat shock response, showing that Aβ fusions promote stress in cells and supporting the notion that intracellular Aβ is a toxic molecule. These studies have relevance in understanding the role of Aβ in the death of neuronal cells, and indicate that yeast may be a new tractable model system for the screening for inhibitors of the stress caused by Aβ.