Open AccessRsc14‐controlled expression of MNN6, MNN4 and MNN1 regulates mannosylphosphorylation of Saccharomyces cerevisiae cell wall mannoproteins
Author(s) -
Conde Raúl,
Cueva Rosario,
Larriba Germán
Publication year - 2007
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2007.00278.x
Subject(s) - saccharomyces cerevisiae , biology , cell wall , yeast , cell , microbiology and biotechnology , biochemistry
ybl006c Δ was selected using an Alcian‐blue‐based screen aimed to identify nonessential genes involved in the regulation of mannosylphosphorylation. When cells of this deletant were mixed with the cationic dye Alcian blue in a typical assay, they remained white, indicating a low number of mannosylphosphate groups on the cell surface. ybl006c Δ cells did not show any defect in growth rate nor in the glycosylation or secretion rate of the major exoglucanase Exg1. Transcriptome analysis of ybl006c Δ using macroarrays showed at least two‐fold changes in the expression of 52 genes (<0.9% of the genome). Three of these have previously been reported to be directly ( MNN6 and MNN4 ) or indirectly ( MNN1 ) implicated in the transfer of mannosylphosphate to the N ‐ and O ‐oligosaccharides. Alterations in the expression of these genes were confirmed by Northern analysis. YBL006C product was recently identified as a subunit (Rsc14) of the RSC chromatin‐remodelling complex of Saccharomyces cerevisiae . It follows that remodelling of chromatin can be an important regulatory mechanism for the maturation of cell wall mannoproteins.