Open AccessA comparative study of peroxisomal structures in Hansenula polymorpha pex mutants
Author(s) -
Koek Anne,
Komori Masayuki,
Veenhuis Marten,
Van Der Klei Ida J.
Publication year - 2007
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2007.00261.x
Subject(s) - biology , peroxisome , biogenesis , mutant , gene , biochemistry , genetics
In a recent study, we performed a systematic genome analysis for the conservation of genes involved in peroxisome biogenesis ( PEX genes) in various fungi. We have now performed a systematic study of the morphology of peroxisome remnants (‘ghosts’) in Hansenula polymorpha pex mutants ( pex1–pex20 ) and the level of peroxins and matrix proteins in these strains. To this end, all available H. polymorpha pex strains were grown under identical cultivation conditions in glucose‐limited chemostat cultures and analyzed in detail. The H. polymorpha pex mutants could be categorized into four distinct groups, namely pex mutants containing: (1) virtually normal peroxisomal structures ( pex7 , pex17 , pex20 ); (2) small peroxisomal membrane structures with a distinct lumen ( pex2 , pex4 , pex5 , pex10 , pex12 , pex14 ); (3) multilayered membrane structures lacking apparent matrix protein content ( pex1 , pex6 , pex8 , pex13 ); and (4) no peroxisomal structures ( pex3 , pex19 ).