Open AccessFormaldehyde dehydrogenase from the recombinant yeast Hansenula polymorpha : isolation and bioanalytic application
Author(s) -
Demkiv Olha M.,
Paryzhak Solomiya Ya.,
Gayda Galina Z.,
Sibirny Volodimir A.,
Gonchar Mykhailo V.
Publication year - 2007
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2007.00255.x
Subject(s) - formaldehyde dehydrogenase , biology , recombinant dna , yeast , formaldehyde , biochemistry , clone (java method) , enzyme , dehydrogenase , strain (injury) , nad+ kinase , dna , gene , anatomy
A recombinant yeast clone, a derivative of the recipient Hansenula polymorpha strain NCYC 495, was chosen as an NAD and glutathione‐dependent formaldehyde dehydrogenase overproducer. Optimal cultivation conditions for the highest yield of enzyme were established. A simple scheme for the isolation of formaldehyde dehydrogenase from the recombinant strain was proposed, and some characteristics of the purified enzyme were studied. An enzymatic method for formaldehyde assay based on formaldehyde dehydrogenase was developed and used for testing real samples.