Open AccessPichia cecembensis sp. nov. isolated from a papaya fruit ( Carica papaya L., Caricaceae )
Author(s) -
Bhadra Bhaskar,
Sreenivas Rao R.,
Naveen Kumar N.,
Chaturvedi Preeti,
Sarkar Partha K.,
Shivaji S.
Publication year - 2007
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2007.00215.x
Subject(s) - carica , biology , botany
The ascogenous yeast YS16 T was isolated from a decaying papaya fruit. Phenotypic traits such as multilateral budding, spheroidal or elongate shape, pseudohyphae formation, asci with one or more ascospores, ability to ferment d ‐glucose, inability to assimilate nitrate and the presence of Q7 ubiquinone suggest its affiliation to the genus Pichia . The nearest phylogenetic neighbor, based on D1/D2 domain sequence of the 26S rRNA gene and ITS region sequence, was identified as Issatchenkia orientalis (NRRL Y‐5396 T , a synonym of Pichia kudriavzevii ) with similarities of 98.2% and 97% respectively. In addition to the difference in the D1/D2 and ITS region sequence, YS16 T differs from I. orientalis with respect to a number of phenotypic traits. However, in the phylogenetic analysis, YS16 T showed close relatedness to the P. membranifaciens clade. Thus, it is proposed to assign the status of a new species to YS16 T , for which the name P. cecembensis sp. nov. is proposed. The type strain of P. cecembensis sp. nov. is YS16 T (=NRRL Y‐27985 T =JCM 13873 T =CBS 10445 T ).