Open AccessAn atypical active cell death process underlies the fungicidal activity of ciclopirox olamine against the yeast Saccharomyces cerevisiae
Author(s) -
Almeida Bruno,
SampaioMarques Belém,
Carvalho Joana,
Silva Manuel T.,
Leão Cecília,
Rodrigues Fernando,
Ludovico Paula
Publication year - 2007
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2006.00188.x
Subject(s) - biology , saccharomyces cerevisiae , programmed cell death , tunel assay , cell cycle checkpoint , microbiology and biotechnology , population , apoptosis , reactive oxygen species , cell growth , biochemistry , cell cycle , yeast , demography , sociology
Ciclopirox olamine (CPO), a fungicidal agent widely used in clinical practice, induced in Saccharomyces cerevisiae an active cell death (ACD) process characterized by changes in nuclear morphology and chromatin condensation associated with the appearance of a population in the sub‐G 0 /G 1 cell cycle phase and an arrest delay in the G 2 /M phases. This ACD was associated neither with intracellular reactive oxygen species (ROS) signaling, as revealed by the use of different classes of ROS scavengers, nor with a terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL)‐positive phenotype. Furthermore, CPO‐induced cell death seems to be dependent on unknown protease activity but independent of the apoptotic regulators Aif1p and Yca1p and of autophagic pathways involving Apg5p, Apg8p and Uth1p. Our results show that CPO triggers in S. cerevisiae an atypical nonapoptotic, nonautophagic ACD with as yet unknown regulators.