Open AccessN‐terminal extension of Saccharomyces cerevisiae translation termination factor eRF3 influences the suppression efficiency of sup35 mutations
Author(s) -
Volkov Kirill,
Osipov Kirill,
Valouev Igor,
IngeVechtomov Sergey,
Mironova Ludmila
Publication year - 2007
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2006.00176.x
Subject(s) - saccharomyces cerevisiae , biology , terminal (telecommunication) , genetics , release factor , translation (biology) , mutant , domain (mathematical analysis) , extension (predicate logic) , transfer rna , eukaryotic translation , yeast , gene , rna , messenger rna , computer science , mathematical analysis , mathematics , programming language , telecommunications
The eukaryotic translation termination factor eRF3 stimulates release of nascent polypeptides from the ribosome in a GTP‐dependent manner. In most eukaryotes studied, eRF3 consists of an essential, conserved C‐terminal domain and a nonessential, nonconserved N‐terminal extension. However, in some species, this extension is required for efficient termination. Our data show that the N‐terminal extension of Saccharomyces cerevisiae eRF3 also participates in regulation of termination efficiency, but acts as a negative factor, increasing nonsense suppression efficiency in sup35 mutants containing amino acid substitutions in the C‐terminal domain of the protein.