Open AccessNatural hybrids from Saccharomyces cerevisiae , Saccharomyces bayanus and Saccharomyces kudriavzevii in wine fermentations
Author(s) -
González Sara S.,
Barrio Eladio,
Gafner Jürg,
Querol Amparo
Publication year - 2006
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2006.00126.x
Subject(s) - saccharomyces , biology , saccharomyces cerevisiae , wine , internal transcribed spacer , hybrid , fermentation in winemaking , genetics , yeast , ribosomal rna , gene , botany , food science
Several wine isolates of Saccharomyces were analysed for six molecular markers, five nuclear and one mitochondrial, and new natural interspecific hybrids were identified. The molecular characterization of these Saccharomyces hybrids was performed based on the restriction analysis of five nuclear genes ( CAT8 , CYR1 , GSY1 , MET6 and OPY1 , located in different chromosomes), the ribosomal region encompassing the 5.8S rRNA gene and the two internal transcribed spacers, and sequence analysis of the mitochondrial gene COX2 . This method allowed us to identify and characterize new hybrids between Saccharomyces cerevisiae and Saccharomyces kudriavzevii , between S. cerevisiae and Saccharomyces bayanus , as well as a triple hybrid S. bayanus × S. cerevisiae × S. kudriavzevii . This is the first time that S. cerevisiae × S. kudriavzevii hybrids have been described which have been involved in wine fermentation.