Open AccessThe mechanism by which overexpression of Gts1p induces flocculation in a FLO8 ‐inactive strain of the yeast Saccharomyces cerevisiae
Author(s) -
Shen Hua,
Iha Hidekatsu,
Yaguchi Soichi,
Tsurugi Kunio
Publication year - 2006
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2006.00086.x
Subject(s) - saccharomyces cerevisiae , repressor , activator (genetics) , biology , yeast , gene , strain (injury) , mutant , reporter gene , flocculation , rna polymerase ii , microbiology and biotechnology , genetics , biochemistry , gene expression , promoter , chemistry , organic chemistry , anatomy
GTS1 induces flocculation when overexpressed in the Saccharomyces cerevisiae strain W303‐1A, which carries a mutant FLO8 , the activator of flocculin genes. Herein, we report that the GTS1 ‐induced flocculation was flocculin‐dependent in nature and was caused by expression of the major flocculin Flo1p. Gts1p bound to the repressor Sfl1p, and their interaction at the transcriptional level was shown by reporter gene assays using the FLO1 promoter, suggesting that Gts1p induces the expression of FLO1 by inhibiting Sfl1p. Furthermore, the Q‐rich domain with the preceding 18 amino acids of Gts1p bound mediators for RNA polymerase II.