Open AccessHeterologous protein production in the yeast Kluyveromyces lactis
Author(s) -
Van Ooyen Albert J.J.,
Dekker Peter,
Huang Michael,
Olsthoorn Maurien M.A.,
Jacobs Denise I.,
Colussi Paul A.,
Taron Christopher H.
Publication year - 2006
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2006.00049.x
Subject(s) - kluyveromyces lactis , biology , kluyveromyces , heterologous , yeast , chymosin , proteomics , heterologous expression , computational biology , saccharomyces cerevisiae , microbiology and biotechnology , biochemistry , enzyme , gene , recombinant dna
Kluyveromyces lacti s is both scientifically and biotechnologically one of the most important non‐ Saccharomyces yeasts. Its biotechnological significance builds on its history of safe use in the food industry and its well‐known ability to produce enzymes like lactase and bovine chymosin on an industrial scale. In this article, we review the various strains, genetic techniques and molecular tools currently available for the use of K. lactis as a host for protein expression. Additionally, we present data illustrating the recent use of proteomics studies to identify cellular bottlenecks that impede heterologous protein expression.